WebAug 13, 2024 · Xu et al. in 2016 [ 45] mutated three rice genes GW2, GW5, and TGW6 to increase seed size by up to 30% in the triple mutant lines. Both durum and bread wheat have also been the subject of successful CRISPR/Cas9-mediated genetic modification for powdery mildew resistance and other objectives [ 42, 46, 47, 48 ]. WebAug 29, 2016 · Thermo-sensitive male sterility hybrid system (TGMS) was started in 1987 in China (Zhang et al., 2016), and in China almost 71% of cultivars are generated by using TMS5 mutants in the twoline...
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WebFeb 18, 2024 · TMS5, a member of RNase Z cleavages the Ub L40 mRNAs, plays an important role in two-line hybrid rice. Here, we identified a new TGMS mutant 93-11s, … WebAug 2, 2006 · In accordance with the sequence information of the tms5 gene region in rice genome, a total of 41 SSR (http://www.gramene.org), 83 STS, and one CAPs marker were … nintendo switch bluetooth audio pc
Environment‐sensitive genic male sterility in rice and other plants ...
The open reading frame of TMS5 was amplified and fused with the N terminus of GFP43 in the pUC18 vector under the control of the CaMV35S promoter. Protoplasts from leaf sheaths of rice plants were isolated as described previously44. Briefly, for protoplast transformation, 10 μl of plasmid carrying TMS5 and … See more Rice accessions, including AnnongS-1 (O. sativa ssp. indica, AnS-1, tms5), AnnongN (O. sativa ssp. indica, AnN, WT siblings of AnS-1), Zhonghua11 … See more Rice florets were photographed with an OLYMPUS DP70 digital camera and an OLYMPUS SZX10 dissecting microscope. Mature pollen grains were stained with 1% I2–KI solution and … See more For functional complementation tests, a 5.3-kb DNA fragment containing 2.3 kb of upstream sequence, the entire TMS5-coding sequence, and … See more The F2 mapping population was generated from crosses between Xiang125S (derived from AnS-1) and Jingxian 89 (O. sativa ssp. indica) and grown in the field during the summer in Guangzhou (DAT>28 °C), Guangdong province … See more WebTargeted mutation of TMS5, Pi21, and Xa13 to develop TGMS rice with enhanced disease resistance ( A) Schematic diagram of TMS5, Pi21, and Xa13 gene structure and gRNA target sites on their genomic sequences. Introns are shown as lines and exons are shown as blue boxes. Targeted sites are labeled with red arrowheads. WebJul 2, 2024 · A collection of 118 male sterile rice lines and 13 conventional breeding lines from the major rice growing regions of China was evaluated and screened against the photosensitive (pms3) and temperature sensitive male sterility (tms5) genes. ... P2 possessing tms5, P3 possessing both pms3 and tms5 genes, and P4 containing … number 7 bus timetable wellington