WebFeb 1, 2024 · RDC data were acquired by NMR spectroscopy for this protein in Phage and stretched Poly Acrylamide Gel (PAG) alignment media. The resulting two sets of RDCs … RDC measurement provides information on the global folding of the protein or protein complex. As opposed to traditional NOE based NMR structure determinations, RDCs provide long distance structural information. It also provides information about the dynamics in molecules on time scales … See more The residual dipolar coupling between two spins in a molecule occurs if the molecules in solution exhibit a partial alignment leading to an incomplete averaging of spatially anisotropic dipolar couplings. Partial molecular … See more The secular dipolar coupling Hamiltonian of two spins, $${\displaystyle I}$$ and $${\displaystyle S,}$$ is given by: where See more • Magnetic dipole–dipole interaction • Residual chemical shift anisotropy (rCSA) • Solid-state nuclear magnetic resonance (ssNMR) See more NMR spectroscopy in partially oriented media was first discovered in 1963, and in a very fundamental paper Alfred Saupe was also able to present the essential theory to describe and understand the observable phenomena only one year later. After this initiation a flood of … See more Any RDC measurement in solution consists of two steps, aligning the molecules and NMR studies: Methods for aligning molecules For diamagnetic molecules at moderate field strengths, molecules have little preference in … See more Books: • Emsley, J. W.; Lindon, J. C. NMR Spectroscopy using liquid crystal solvents; Pergamon Press: Oxford, U.K., 1975. Review papers: See more
Residual dipolar coupling - Wikipedia
WebResidual dipolar couplings (RDCs) are an efficient tool for the determination of the relative configuration of small organic molecules.1–5The use of RDCs has been boosted by the availability of weak aligning media compatible with standard organic solvents such as CDCl36–10or DMSO-d6,11–18and most other common NMR solvents.19–22While it is … WebDec 14, 2024 · As will be discussed in the ‘Origins and manifestation of anisotropic NMR parameters’ section, RDC and RCSA are the residual manifestations of the prominent … fly high vk
REDCRAFT: A computational platform using residual …
WebMay 1, 2024 · Residual dipolar couplings (RDCs) are powerful nuclear magnetic resonance (NMR) probes for the structure calculation of biomacromolecules. Typically, an alignment tensor that defines the orientation of the entire molecule relative to the magnetic field is determined either before refinement of individual bond vectors or simultaneously with this … WebThe development of residual dipolar coupling (RDC) in protein NMR spectroscopy, over a decade ago, has become a useful and almost routine tool for accurate protein solution … WebHere we present a residual dipolar coupling (RDC)-based NMR approach to characterizing the molecular recognition of CaM with five different CaM kinases. Our analyses indicate that CaM kinase I and likely IV use the same CaM binding mode as myosin light chain kinase (1-14 motif), distinct from those of CaM kinase II (1-10 motif) and CaM kinase ... green led christmas lights white wire