Phospho-kinase antibody array

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August undefined, 2024

WebAntibody Arrays; IHC/Pathology Ab. IHC Ab; Pathology Ab; Circulating Rare Cell Ab. ... (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429. Form: ... The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal ... WebJul 9, 2024 · Methods Using a phospho-kinase antibody array we identified changes in signalling associated with HIP1 overexpression PNT1 cells. For validation Western blots were used together with knockdown or inhibitor treatments and phenotypic assays for cellular transformation. The cell line was xenografted to assess tumour growth.

Phospho Explorer Antibody Array - Full Moon BioSystems

WebPhospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse … WebSep 20, 2024 · The phospho-protein arrays used in our experiments are based on analysis of tissue samples on nitrocellulose membranes, where specific antibodies against selected … ready to go aberdeenshire

Proteome Profiler Human Phospho-RTK Array Kit by Bio-Techne

WebR&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. WebProtein kinase. A protein kinase is a kinase which selectively modifies other proteins by covalently adding phosphates to them ( phosphorylation) as opposed to kinases which … WebThe Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 37 kinase phosphorylation sites and 2 related total proteins without performing numerous immunoprecipitations and Western blots. ... Each capture antibody was carefully selected using cell lysates prepared … ready to fix meals

ABL1/ABL2 (phospho Y393/Y429) polyclonal antibody

7 Ways to Study Protein Phosphorylation

WebThe newly updated T-Cell Receptor Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein expression profiling. It is suitable for comparing protein expressions between non-treated and treated samples and identifying candidate biomarkers related to TCR signaling. WebHuman Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. In this technology, capture … ready to give up memeWebThe array kit allows for the simultaneous detection of 28 receptor tyrosine kinases and 11 important signaling nodes, when phosphorylated at tyrosine or other residues. Target-specific capture antibodies have been spotted in … ready to go chords

"WebApr 11, 2024 · Human Phospho-Kinase Array - Eight nitrocellulose membranes; Part A contains 21 antibodies printed in duplicate, and Part B contains 18 antibodies printed in … " - Phospho-kinase antibody array

Phospho-kinase antibody array

Phospho-tyrosine targets for treating esophageal cancer OTT

WebJun 18, 2024 · Here, we conducted integrated analysis of human phospho-kinase antibody array, transcriptome analysis with RNA-seq, and proteomic analysis of protein pulldown to study the molecular detail of PSPC1-potentiated phenotypical transformation, adhesion, and motility in human hepatocellular carcinoma (HCC) cells. We found that PSPC1 … WebFeb 28, 2024 · To validate the kinase array phospho-Y2H results, AAR2 and PRPF8 were tested in a co-expression and immunoprecipitation assay in HEK293 cells. Co-expression of either SGK2 or CK2α1 reduced the amount of wild-type AAR2 co-immunoprecipitated with PRPF8 in comparison to the nonphosphorylatable mutant S284A (Fig 5F). Together, these …

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WebMay 11, 2024 · An antibody specific to the phosphorylation-of-interest is added to the membrane. The phosphorylation antibody is then bound by a secondary antibody conjugated to horseradish peroxidase (HRP). Antibody binding is detected when an HRP substrate is added, resulting in chemiluminescence. Web40 rows · Each Human Phospho-Kinase Antibody Array Kit contains most of the …

WebProteome Profiler Human Phospho-RTK Array Kit contains 4 membranes - each spotted in duplicate with 42 different RTK antibodies. The R&D Human Phospho-Receptor Tyrosine Kinase (Phospho-RTK) Array is a rapid, sensitive, and economical tool used to detect changes in phosphorylation between samples. R&D Systems Human Phospho-Receptor … WebProduct name Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) Sample type Tissue Extracts, Cell Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human Product overview ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors.

WebNational Center for Biotechnology Information WebR&D Systems™ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments.

WebCapture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each …

WebHuman Phospho AKT Pathway Protein Array, 18 Target proteins (HA-PAA-PA2) Human Phospho AKT Pathway Protein Array, 18 Target proteins (HA-PAA-PA2) ... AMPKa (Thr172), S6 Ribosomal Protein (Ser235/236), mTOR (Ser2448), HSP27 (Ser78), Bad (Ser112), p70 S6 Kinase (Thr389), PRAS40 (Thr246), p53 (Ser15), p38 (Thr180/Tyr182), SAPK/JNK … how to take minutes meetingWebThe PathScan ® Intracellular Signaling Array Kit (Chemiluminescent Readout) is a slide-based antibody array founded upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 18 important and well-characterized signaling molecules when phosphorylated or cleaved. ... Akt is a protein kinase generally ... ready to go auto insuranceWebJan 9, 2024 · Phospho-proteomic analyses revealed that, while not impacting on the global tyrosine phospho-proteome in colon cancer cells, this region modulates phosphorylation of specific membrane-localized... ready to glare weddingWebHuman RTK Phosphorylation Antibody Array -Membrane (71 Targets) Sample type Cell Lysate, Tissue Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human … how to take minutes in a meeting uk templateWebRayBiotech: Empowering Your Proteomics how to take mirror closet doors offWebA membrane-based antibody array for the parallel determination of the relative levels of human protein kinase phosphorylation. Validated for analyte detection in cell lysates. Troubleshooting Guide Key Benefits Detects phosphorylation of 37 human kinases and total amounts of 2 related proteins simultaneously Requires no specialized equipment ready to go appetizersWebJan 19, 2024 · Using a human phospho-kinase antibody array, Akt1/2 signaling, known to regulate Nanog, was found to be highly activated in sarcoma spheroid cells compared with monolayer cells. how to take minutes of meetings effectively