Lamp2a sirna

Author: ztti

August undefined, 2024

TīmeklisSynthetic peptide corresponding to Human LAMP2A aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) 阳性对照 WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. Tīmeklis2024. gada 1. okt. · Transient transfection efficiency of Lamp2A siRNA was evaluated by RT-PCR and immunoblot analysis after the cells were transfected with LAMP2A siRNA for various times (Fig. 5C–D). The results showed that LAMP2A inhibition resulted in a further 52.1 % increase of α-syn oligomers ( Fig. 5 E), and a 33.6 % …

Frontiers The Lysosomal Membrane Protein Lamp2 Alleviates …

Tīmeklis2024. gada 21. marts · LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. Diseases associated with LAMP2 include Danon Disease and Left Ventricular Noncompaction . Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System . Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart … shortness of breath triage questions

Full article: LAMP2A overexpression in breast tumors promotes …

TīmeklissiRNA. 48 h later, cells were lysed and assayed for ASYN and Lamp2a levels. Non transfected cells were used as a control (ctrl). Representative immunoblots of … Tīmeklis2012. gada 7. febr. · Next, we examined whether lysosomal translocation of GAPDH-HT would occur in HeLa cells with siRNA-mediated knockdown of LAMP2A. We … Tīmeklis2024. gada 7. febr. · Lamp2 is a heavily glycosylated type-1 lysosomal membrane protein and an important regulator of autophagy. There are three different isoforms in humans and mice and only one isoform in … santa anita brunch at the races

Establishment of a Novel Fluorescence-Based Method to …

In Vivo RNAi Thermo Fisher Scientific - KR

TīmeklisA, transient transfection of rat Lamp2a siRNA (L1), effectively down-regulates endogenous Lamp2a but has no effect on the steady state levels of overexpressed WT ASYN. PC12 cells expressing WT … santa and the north poleTīmeklis2016. gada 5. jūl. · LAMP2A is a lysosomal membrane protein and a lysosomal receptor for CMA substrate (Kaushik & Cuervo 2012 ). TSG101 is a subunit of ESCRT‐I complex, which is involved in the biogenesis of MVBs (Hurley 2008 ). TSG101 is reported to participate in the incorporation of mA substrate to late endosomes (Sahu et al. 2011 ). shortness of breath va disability rating

"Tīmeklis2024. gada 8. febr. · LAMPs are essential to maintain lysosomal integrity and functionality and are also involved in other cellular functions, such as autophagy 3. … " - Lamp2a sirna

Lamp2a sirna

p38-TFEB pathways promote microglia activation through inhibiting CMA ...

Tīmeklis2024. gada 24. nov. · Lysosomal p38 MAPK directly phosphorylates the CMA receptor LAMP2A at T211 and T213, which causes its membrane accumulation and active … Tīmeklis2024. gada 10. marts · D Silencing LAMP2A in HeLa cells by siRNA for 24, 30, 36, 42, and 48 h increases the expression level of SMAD3 (quantification shown at right; n = 3, **P <0.01, ***P <0.001 vs negative control cells at the same time point). E The level of SMAD3 also increases in U251 cells after LAMP2A is silenced by siRNA.

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Tīmeklis2024. gada 20. dec. · Then the substrate/chaperon complex binds the lysosome membrane-bound LAMP2A, which triggers the LAMP2A multimerization and followed lysosomal internalization of substrate proteins for degradation. The reduced neuronal CMA activity is observed in the aging brain and neurodegenerative diseases. Tīmeklis暨南大学,数字图书馆. 开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆

Tīmeklis2024. gada 1. dec. · Compensation effect of macroautophagy by CMA inhibition. (A) SH-SY5Y cells were transfected with Lamp2A siRNA or scrambled control siRNA for 36 h and then treated with 100 μM of DQ for 24 h. Western blot analysis of LC3II, cleaved caspase-3, cleaved PARP and phospho-α-synuclein (p-α-syn) expressions in whole … TīmeklisGene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of LAMP-2 gene silencing results, individual duplex components or plasmids are also available upon request. Suitable control antibody: LAMP-2 Antibody (H4B4): sc-18822 is recommended as control …

Tīmeklis2012. gada 9. dec. · a KYSE30 cells were transfected with control siRNA (80 nmol/L) or PKCι siRNA (80 nmol/L), and at 36 h after transfection, the cells were treated without or with H 2 O 2 (60 μmol/L) for an additional 12 h. The cells were then harvested for Annexin-V/PI staining and flow cytometry analysis. Tīmeklis2024. gada 21. jūl. · Lysosomal associated membrane protein 2A (Lamp2a), a lysosomal receptor, mediates the translocation of CMA substrates into the lysosomal lumen, a process that is assisted by a luminal resident form of Hsc70 (Cuervo and Dice 1996; Agarraberes et al. 1997 ). The mechanistic regulation of CMA is not fully …

Tīmeklis1.1 主要实验材料人胚胎肾细胞293T 及Atg3 基因敲除的293T 细胞、质粒pXBP1u-Luc、pCS-H5HA由本实验室保存；GFP-250 质粒由Elizabeth Sztul 惠赠；表达伪狂犬病毒(PRV) gE 蛋白的重组质粒PRV-gE 及鼠源抗gE 蛋白单克隆抗体(MAb)由田志军研究员惠赠；Lamp2a siRNA 购自GenePharma 公司 ...

TīmeklisA. The quantification of the colocalization between a lysosomal protein such as LAMP2 and LC3 could be used via a standard immunofluorescence protocol (fixation using … santa anita craft beer festivalTīmeklisAvailability. Inventoried. Catalog #. AM16708. Standard 5 nmol. Purification: HPLC In-Vivo Ready Standard. Size: 5 nmol 20 nmol 20 nmol 20 nmol 20 nmol 40 nmol 40 … shortness of breath treatment medicineTīmeklis2024. gada 21. marts · LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. Diseases associated with LAMP2 include Danon Disease and Left … shortness of breath treatment planTīmeklis2024. gada 5. febr. · EGLN3 and LAMP2A siRNAs (50–75 nM) were transfected into cells using Lipofectamine 2000 reagent (Invitrogen) as described previously . siRNA transfection efficiency was verified by ... shortness of breath upon movementTīmeklisIn order to confirm the specificity of the Lamp2a and Lamp2b siRNAs, an RT-qPCR was performed in 72-h siRNA-treated samples using primers specific to both isoforms. shortness of breath treatment home remediesTīmeklis2016. gada 5. jūl. · (A) Immunoblot analyses of siRNA‐mediated knockdown of LAMP2A and TSG101 in Atg5‐KO MEF cells. Cells were transiently transfected with a plasmid … shortness of breath va claimTīmeklis2012. gada 7. febr. · Numbers of GAPDH-HT dots were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t-test, n = 30). shortness of breath vomiting fatigue