Webaffi nity (C). In this experiment, recombinant human PD-L1/B7-H1 His-tag Avi-tag biotinylated protein was captured at a low coupling density to the active fl ow cell via the Avi-tag biotin. Then, recombinant PD-1 His-tag protein at a concentration range between 3.2 nM and 13.2 uM was passed over both active and uncoupled reference fl ow cells WebAntibodies that detect 6x His Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Immunohistochemistry. Our 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep …
Biotin-Anti-His-Tag Antibody - Thermo Fisher Scientific
WebAnti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western ... WebA DNA sequence encoding the Mouse FLT1 (NP_034358.2) (Met1-Glu759) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. iss bowl league sheet
Recombinant Protein A/G, His-tag(重组蛋白A/G, His-tag)(P5043 …
WebNov 15, 2007 · We have developed an approach for site-specific reversible biotinylation of recombinant proteins through their histidine tag using biotin conjugated to the … WebIn a functional flow cytometry test, (A) Recombinant SARS-CoV-2 Spike His-tag Biotin Protein (Catalog # BT10549; 500 ng/ml) binds to HEK293 human embryonic kidney cell line transfected with recombinant human … WebKeywords: His-tagged protein, Ni-NTA, biolayer interferometry, avidin-biotin, norovirus, non-labelled detection 1. Introduction Histidine tags, e.g. insertion of a stretch of 5-10 histidine amino acids in either the N- or C-terminus of a recombinant protein, are typically used as affinity tags for purification purposes [1-3]. idiographic research example